Anti-PLK1 (phospho T210) antibody [2A3] ab39068 from Abcam

Product Specs
ItemAnti-PLK1 (phospho T210) antibody [2A3]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab39068
Quantity100 UG
ApplicationsWestern blot
IsotypeIgG1
HostMouse, Nude mouse
Antibody TypePrimary
ClonalityMonoclonal
Conjugate/TagUnconjugated
Clone2A3
Formatab39068 is an affinity purified IgG
Concentration0.5 mg/ml
ModificationsPhospho-T210
Gene NamePLK1
ReactivityHuman
TargetPLK1

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomesNucleic acids researchJune 9, 2023Leem J, Kim JS, Oh JS.anti-CENP-A (1:200, 2048S, Cell Signaling), anti-HEC1 (1:250, ab3613, Abcam) anti-p-T210-PLK1 (1:250, ab39068, Abcam), anti-γ-tubulin (1:250, ab11316, Abcam), and anti-GFP (1:500, ab1218, Abcam). After being washed

(2) Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in DrosophilaNature CommunicationsDecember 1, 2022Shuo Yang, Jennifer McAdow, Yingqiu Du, Jennifer Trigg, Paul H. Taghert, Aaron N. Johnson(1:1000, gift from R. Cripps), α-Tropomyosin (1:600, Abcam, MAC141), α-PLK1-phospho-T210 (1:100, Abcam, ab39068), α-GFP (1:600, Torrey Pines Biolabs, TP-401), α-GFP (1:300, Aves Labs, GFP-1020), α-dsRed (1:300, Takara,

(3) Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lambliaThe Korean Journal of ParasitologyJune 30, 2022Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung Parkalbumin, the cells were incubated with 1:100 diluted anti-GlKin-13 antibodies or anti-phosphorylated PLK (ab39068; Abcam, Cambridge, Massachusetts, USA) overnight at 4°C, and subsequently incubated with 1:200 diluted

(4) CCAR2 controls mitotic progression through spatiotemporal regulation of Aurora BCell death & diseaseJune 7, 2022Ryu J, Kim JE.(Santa Cruz Biotechnology; sc-293127), PLK1 (Santa Cruz Biotechnology; sc-55504), PLK1-pT210 (Abcam, ab39068), BubR1 (BD Biosciences, 612503). BubR1-pS670 antibody was provided by C.W. Lee (Sungkyunkwan University).

(5) The spindle assembly checkpoint and the spatial activation of Polo kinase determine the duration of cell division and prevent tumor formationPLOS GeneticsApril 4, 2022Emmanuel Gallaud, Laurent Richard-Parpaillon, Laetitia Bataillé, Aude Pascal, Mathieu Métivier, Vincent Archambault, Régis GietAbcam 195172), anti-Cnn (rabbit, 1:500, [58]) and monoclonal anti-phospho-Plk1T210 (mouse, 1:500, Abcam ab39068). Secondary antibodies: anti-rat, anti-mouse and anti-rabbit conjugated to 488, 568, 647 Alexa-Fluor

(6) A polo-like kinase modulates cytokinesis and flagella biogenesis in Giardia lambliaParasites & VectorsDecember 1, 2021Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung ParkMolecular Probes). Antibodies specific to the phosphorylated form of PLK were purchased from Abcam (ab39068; Cambridge, MA, USA) and then used for IFA of G. lamblia cells (1:100) along with anti-HA antibodies

(7) An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errorsCell reportsNovember 9, 2021Orr B, De Sousa F, Gomes AM, Afonso O, Ferreira LT, Figueiredo AC, Maiato H.D2C8),Cell Signaling Technology,Cat#3377; RRID:AB_1549592 mouse anti-pPLK1 T210 (Monoclonal),ABCAM,Cat# ab39068; RRID:AB_10861033 mouse anti-GAPDH (Monoclonal),Proteintech,Cat#10494-1-AP; RRID:AB_2263076 Human

(8) Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregationCell reportsJuly 13, 2021Xu L, Ali M, Duan W, Yuan X, Garba F, Mullen M, Sun B, Poser I, Duan H, Lu J, et al.anti-Bora,Cell Signaling Technology,Cat#12109; RRID:AB_2797821 Mouse monoclonal anti-pT210-PLK1,Abcam,Cat#Ab39068; RRID:AB_10861033 Goat polyclonal anti-PP1β,Santa Cruz,Cat#Sc-373782; RRID:AB_10916703 Goat polyclonal

(9) Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assemblyThe Journal of cell biologyFebruary 1, 2021Chinen T, Yamazaki K, Hashimoto K, Fujii K, Watanabe K, Takeda Y, Yamamoto S, Nozaki Y, Tsuchiya Y, Takao D, et al.α-tubulin (T5168, IF 1:1,000; Sigma-Aldrich), HSP90 (610419, WB 1:5,000; BD Biosciences), and pT210-PLK1 (ab39068, IF 1:300; abcam). Fluorescein isothiocyanate–labeled anti-GFP was purchased from Abcam (ab6662, IF

(10) Plk1-dependent cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motifJournal of Cell ScienceNovember 6, 2019Diana Papini, Xavier Fant, Hiromi Ogawa, Nathalie Desban, Kumiko Samejima, Omid Feizbakhsh, Bilge Askin, Tony Ly, William C. Earnshaw, Sandrine Ruchaudfrom Sigma and Upstate Biotech (now Merck-Millipore), respectively, and anti-Plk1 pT210 from Abcam (ab39068, 1:500). Rabbit polyclonal (WCE1186, 1:500) and mouse monoclonal anti-INCENP (3D3, 1:500) were previously

(11) Plk1 overexpression induces chromosomal instability and suppresses tumor developmentNature communicationsAugust 1, 2018de Cárcer G, Venkateswaran SV, Salgueiro L, El Bakkali A, Somogyi K, Rowald K, Montañés P, Sanclemente M, Escobar B, de Martino A, et al.primary antibodies against: FLAG (1:500, Sigma F7425), Plk1 (1:2)68, Plk1-phospho-T210 (1:200, Abcam ab39068), Pericentrin (1:3000, Abcam ab4448), γ-Tubulin (1:1000, Sigma T6557), Aurora B-phospho-T232 (1:500,

(12) Adducin-1 is essential for spindle pole integrity through its interaction with TPX2EMBO reportsAugust 1, 2018Hsu WH, Wang WJ, Lin WY, Huang YM, Lai CC, Liao JC, Chen HC.anti‐FLAG (F3165) antibodies were purchased from Sigma‐Aldrich. The mouse monoclonal anti‐PLK1 pT210 (ab39068), anti‐Aurora‐A (ab13824), anti‐NEDD1 (ab57336), mouse anti‐pericentrin (ab28144), anti‐TPX2 (ab32795),

(13) CDK1 and PLK1 coordinate the disassembly and reassembly of the nuclear envelope in vertebrate mitosisOncotargetJanuary 30, 2018Ines J de Castro, Raquel Sales Gil, Lorena Ligammari, Maria Laura Di Giacinto, Paola Vagnarellianti-atubulin (monoclonal B512, Sigma, T5168) 1:1000; anti-PLK1 (Abcam, ab175808) 1:1000; anti-PLK1ph (Abcam, ab39068) 1:300; anti-INCENP (gift from WC Earnshaw, Edinburgh) 1:500; Mel28 (Gift from M. Platani, Edinburgh)

(14) Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stabilityeLifeNovember 20, 2017Dumitru AMG, Rusin SF, Clark AEM, Kettenbach AN, Compton DA.1:1000);,Bethyl Laboratories,Cat# A300-251A Mouse monoclonal anti-Plk1 pThr210 (IF/WB 1:500);,Abcam,Cat# Ab39068 Mouse monoclonal anti-Securin (WB 1:1000),Abcam,Cat# AB3305 "Mouse monoclonal anti- α-Tubulin (DM1α)

(15) Bcl2l10, a new Tpx2 binding partner, is a master regulator of Aurora kinase A in mouse oocytesCell cycle (Georgetown, Tex.)December 1, 2016Lee SY, Kim EY, Kim KH, Lee KA.

(16) Opposing post-translational modifications regulate Cep76 function to suppress centriole amplificationOncogeneOctober 13, 2016Marine Barbelanne, Andrea Chiu, Jin Qian, William Y. Tsanganti-acetyl lysine (#9441; Cell Signaling); anti-phosphoserine (05-1000X; Millipore); anti-Plk1-pT210 (ab39068; Abcam) and anti-CP110 (A301-344A) and anti-Cep76 (A302-326A) (Bethyl Laboratories) Mass spectrometric

(17) Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidyThe Journal of clinical investigationFebruary 1, 2016Naylor RM, Jeganathan KB, Cao X, van Deursen JM.Sigma-Aldrich); rabbit anti-HA (3724, 1:250; Cell Signaling Technology); mouse anti–p-PLK (Thr210) (ab39068/clone 2A3, 1:300; Abcam); rabbit anti–p-HH3 (Ser10) (06-570, 1:10,000; EMD Millipore); mouse anti-NUP88

(18) Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomesThe Journal of cell biologyJuly 6, 2015Platani M, Trinkle-Mulcahy L, Porter M, Jeyaprakash AA, Earnshaw WC.

(19) Unique subcellular distribution of phosphorylated Plk1 (Ser137 and Thr210) in mouse oocytes during meiotic division and pPlk1(Ser137) involvement in spindle formation and REC8 cleavageCell cycle (Georgetown, Tex.)January 1, 2015Juan Du, Yan Cao, Qian Wang, Nana Zhang, Xiaoyu Liu, Dandan Chen, Xiaoyun Liu, Qunyuan Xu, Wei Ma

(20) Polo kinase regulates the localization and activity of the chromosomal passenger complex in meiosis and mitosis in Drosophila melanogasterOpen BiologyNovember 8, 2014Mar Carmena, Miguel Ortiz Lombardia, Hiromi Ogawa, William C. Earnshawrabbit polyclonal anti-INCENP Rb-801, Rb-803 [25], 1 : 500; mouse monoclonal anti- Plk1T210Ph (Abcam ab39068, 1 : 100) and rabbit polyclonal anti-Histone3Ser10Ph (Upstate, 1 : 500). Secondary antibodies were obtained

Be the First to Write a Review!

Anti-PLK1 (phospho T210) antibody [2A3] from Abcam

Abcam

Citations (30)

Reviews

Anti-PLK1 (phospho T210) antibody [2A3] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map